FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14106132
Sequences flagged as poor quality0
Sequence length86-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG48600.022.774861
TAGATAG16400.020.1890775
TATTAGA39100.018.993242
CGCGTGT46300.018.593731
GTGTTAT71550.018.3785531
TGTGCGT63300.018.084948
TGTTATG73900.017.0260282
AATACGG29700.016.7222675
CTATAGT14300.016.5385064
GTTATGC72950.016.3394553
GCCGTCT96500.015.53123794-95
TTATGCC82950.015.2249964
TATAGTG19050.015.1459725
TTCCGTC75850.014.896275594-95
ATATTAG41950.014.771191
GTATTGG70600.014.6058871
TAAGAGT21250.014.4682734
TAGGCCC22950.014.4270534
TATTAGT44150.014.3565552
TCTATAG17550.014.28436853
Produced by FastQC (version 0.11.5)