FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23938750
Sequences flagged as poor quality0
Sequence length86-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAG21300.026.4332225
GCCGTCT615350.024.30810294-95
CGCGTGT69900.023.0147511
TGTGCGT125100.021.4072978
GTATTAG54800.020.2904571
TGCCGTC793000.019.56047894-95
CCGTCTT500750.017.4840394-95
TAGCCCC53150.017.3585765
GTGTGCG155450.017.287867
GTGCGTC164850.016.7049399
GCTTGAA183650.016.5028294-95
AGGACGC133950.016.1783289
GTATTGG108600.015.5104611
ATGCCGT831300.015.26088592-93
TTAGAAC45750.015.2022893
CGGGACG80050.014.8943645
TATTAGA42300.014.8769842
TATGCCG876550.014.73503192-93
TATCTAG44100.014.5916641
TAATACT42000.014.4192754
Produced by FastQC (version 0.11.5)