FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35729045
Sequences flagged as poor quality0
Sequence length86-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAG25900.025.5809635
GCCGTCT1316850.022.98592494-95
CGCGTGT122450.022.2238351
GCTTGAA361700.021.937294-95
TGTGCGT203450.021.2387268
AGGACGC248150.021.0178159
GTATTAG69250.020.8444271
CTTGAAA284050.020.08100794-95
TGCCGTC1647300.018.82996494-95
GTGTGCG241750.017.814117
GTATTGG157800.017.1853161
GTGCGTC257000.017.1449729
CCGTCTT1060950.016.9138494-95
CGGGACG134650.016.237695
CTCCCGA272200.015.99593451
TAGCCCC71950.015.5885675
CGTGTGG286050.015.0394451
ATGCCGT1757250.014.75221792-93
GTGTGGT298600.014.7083872
GAGTAGG116750.014.2690711
Produced by FastQC (version 0.11.5)