FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35729045
Sequences flagged as poor quality0
Sequence length35-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG412860.11555304654798358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAG30600.025.669125
CGCGTGT99600.024.4298841
CGTATCA42150.020.84339194-95
TGTGCGT187200.020.069618
GTATTAG68500.019.9030461
GTATTGG152150.017.6101111
CGGGACG121700.017.3018995
AGGACGC209400.016.8346429
GTGTGCG225450.016.7065647
GTTATGC84550.016.1177043
GTGCGTC239400.016.0097739
GTGTTAT100750.015.6934711
CTACACT123900.015.581644
TCGGTGG637650.015.594-95
CGGTGGT448300.014.9625994-95
TGTTATG99050.014.8093222
TATTGGA165300.014.1124382
CGGTTCG65500.013.6535264
ATTGGAT149900.013.6050433
CTATAGT26450.013.5960344
Produced by FastQC (version 0.11.5)