FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36955993
Sequences flagged as poor quality0
Sequence length86-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1325900.35877807423548325No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG880060.23813728939714868No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG831460.22498651301292322No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC828730.2242477965617106No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA721360.19519432206841256No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC513170.13885975138051357No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT432380.11699861508253885No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT408130.1104367564957597No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT383320.10372336632924462No Hit
GTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAG377080.10203487158361568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA327500.026.2094822
GTATTAG365050.025.1727071
GCCGTCT778950.023.88470894-95
ATTAGAG396950.021.8021453
TTAGAGG421250.021.0162474
TAGGGTC403250.020.9334964
CTAGGTT89600.020.8598144
TAGAGGC425550.020.659345
CATAGGG413700.020.6225642
CCATAGG433200.019.5741371
AGGCACC444550.019.5115838
GCCCTAA114950.019.4708611
TGTGCGT94750.019.32788
GAGGCAC441800.019.2675617
TAATACT236700.019.1908534
TGCCGTC950650.019.1804894-95
ATAGGGT460800.018.534723
GTATGAA739100.018.0670361
ACCGGTA33500.017.939478
TATGAAT738800.017.8309632
Produced by FastQC (version 0.11.5)