FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36955993
Sequences flagged as poor quality0
Sequence length44-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA1310690.3546623682930127No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG842340.22793055513350702No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC833210.22546004919959803No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG819280.22169070115366676No Hit
GTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA705460.19089190757233881No Hit
CTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACC494730.1338700329334947No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT453210.12263504866450213No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT412890.11172477492351511No Hit
GTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGT378140.10232169921668727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA317450.026.5938822
GTATTAG353850.025.4501171
ATTAGAG385050.022.2076533
TTAGAGG412400.021.1594284
TAGAGGC416650.020.7391535
TAGGGTC408850.020.3824794
CATAGGG422400.020.075962
GAGGCAC428850.019.6526057
AGGCACC435150.019.5420768
CCATAGG436200.019.3319991
GCCCTAA104250.018.984481
TAATACT247350.018.6341114
GTATGAA734050.018.1572991
TATGAAT733650.018.0863652
ATAGGGT473350.017.8650343
GCATAAG156950.017.768571
GGCACCG480250.017.3521639
ATGAATG798800.016.694163
AGAGGCA520550.016.5360556
GTTTAGG264450.016.2928221
Produced by FastQC (version 0.11.5)