FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11083653
Sequences flagged as poor quality0
Sequence length35-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA140840.1270700192436555No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG48600.024.1588861
TGTGCGT45150.021.4816468
TATTAGA44750.021.462212
CGCGTGT25250.020.4374451
CGGTGGT211900.019.30538794-95
TCGGTGG276050.018.40502794-95
TAATACT41250.017.3190654
GTGTGCG58200.016.9900537
GTGCGTC64750.016.5135369
TAGGGTC63450.016.404484
TTAGAAC24300.016.160073
TAGGCCC20850.016.1110444
CATAGGG70050.016.0746022
CGTATCA18300.016.05633594-95
GTATCAT26850.015.61828494-95
ATAATAC9150.015.51213553
CCATAGG73150.014.8211761
GTTAGGT44200.014.6743811
TAGGTAC45600.014.6293723
GTATGAA91100.014.6032471
Produced by FastQC (version 0.11.5)