FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10267684
Sequences flagged as poor quality0
Sequence length46-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA403110.3926007072286214No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT325600.31711143428255095No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG126110.12282224501650031No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG23950.019.3780881
TATCTAG15150.016.8799631
CGCGTGT19550.016.7144721
TGTGCGT38750.016.6117888
TATTAGA26950.016.5090662
GTACTAG10200.015.785891
GTATGAA43400.015.7131441
GTACTAA7800.015.1787411
TAGGGTC33650.015.0504674
TATACTG17400.014.6891215
ATAGGGT36200.014.2517833
TTAGAAC15300.014.230423
TATGAAT41300.014.2110132
GTGCGTC46650.014.2044889
TAATACT22500.014.0943024
CATAGGG40500.013.9073812
TACTAGG11400.013.7013232
GTTTAGG27500.013.6045671
TAGATAG12250.013.5233175
GTCCTAT20450.013.4315151