FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005406575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48281806
Sequences flagged as poor quality0
Sequence length35-101
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGGAGGGCCGCTGCGGTGAGCCTTGAAGCCTAGGGCGCGGGCCCGG616350.12765678234985658No Hit
GCTCCGGGACGGCTGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCC579990.12012599528692029No Hit
CTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAGTTGTT561730.11634403236697483No Hit
GGCGGGGACAGGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCG532990.11039147955650208No Hit
GGCGGGGGACCGGCTATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGT528010.1093600351237897No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG524710.10867654784910076No Hit
CCCGAGGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGAC514290.10651838499993144No Hit
ACCCGGGCCCGCGCCCTAGGCTTCAAGGCTCACCGCAGCGGCCCTCCTAC500950.10375543947134042No Hit
ACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCA491680.10183546158153238No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)