FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22381493
Sequences flagged as poor quality0
Sequence length86-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG226270.10109691967376795No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA56900.021.3690722
GTATTAG75500.021.3204141
TAGATAG19100.016.5961065
CGACCCG79550.016.295785
TTAGAAC53050.016.052843
TAGGGTC86150.015.2670194
TAGCCCC50000.015.0449895
TGTGCGT80500.014.4578598
ATAGGGT92300.014.45480253
TAATACT51850.014.3256944
GTATTGG120100.014.2701741
GTATGAA134250.014.176711
CATAGGG102250.014.0661432
TAGAACT82600.013.9184544
CCGCGCG110950.013.8586459
TATGAAT130500.013.5589442
TAGACCG16150.013.4756615
CTACACT70700.013.3167554
GTCCTAG33600.013.1041041
CTAATAC58200.013.0065383
Produced by FastQC (version 0.11.5)