FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005406579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28324001
Sequences flagged as poor quality0
Sequence length35-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGAATCTCTACCTGTTATCCATCGACTACGCCTCTCGGCCTTGCC548520.19365908086219882No Hit
TTCCTGTACTGTGTCAAATCGTTAAGAGCGAAGGAGGGACGCAGGAGGTG406130.14338722837921097No Hit
GGGTTGGGCTGTTCGCCCATTAAAGCGGCACGCGAGCTGGGTTCAGAACG361080.12748198956778742No Hit
CCTTTACACTCTGCGAATGATTTCCAACCATTCTGAGGGAACCTTTGAGC356490.12586145580209518No Hit
GCCTTAGACGGCTCCTTCCCGAAGGTTAGGCCACCGGCTTTGGGCATTGC356200.1257590691371604No Hit
GGGTAAGTCGGGACCTAAGGCAAGGCCGAGAGGCGTAGTCGATGGATAAC354180.12504589305726968No Hit
GTTGTGGGTAGCGGTGAAATTCCAAACGAACTTGGAGATAGCTGGTTCTC321920.11365625922693619No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG291270.10283504791572348No Hit
ACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGACTTTCTGGTTGA289220.10211128011187401No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)