FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28791609
Sequences flagged as poor quality0
Sequence length86-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA293650.10199152121022483No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG106300.021.4751241
CGACCCG156550.021.1622645
TATTAGA77000.020.8366742
TAGCCCC67600.020.6534425
CGGGACG106650.019.3039235
GTGTTAT164950.018.97891
TGTGCGT118200.018.538768
CGCGTGT63600.018.4678571
CCGCGCG191950.018.4676179
TGTTATG165400.018.0556562
TAGATAG27550.017.6942755
ACCCGCG158350.017.5144587
GTTATGC170050.017.0886963
TAATACT72100.015.8853674
GTGTGCG147000.015.8081597
TTATGCC189000.015.5505924
GGGTATA68950.015.5237311
CGTATCA23100.015.41628794-95
CGGTTCG41000.015.1218399
TTAGAAC76900.015.0169123
Produced by FastQC (version 0.11.5)