FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34270250
Sequences flagged as poor quality0
Sequence length35-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA639080.1864824446859886No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCACGGGCAG467090.13629605853473495No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC420490.122698258693765No Hit
GTCATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTAAG390330.11389762257351492No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA175400.024.8708742
GTATTAG214350.023.7878461
TGTGCGT135550.022.025098
TAGCCCC80450.020.936875
CGGGACG99200.019.55515
CGACCCG171000.019.146875
GCGTAGC85900.018.5069922
TAGGGTC252350.018.2992944
GTGTGCG169500.017.8647967
CATAGGG279700.017.1189752
CGCGTGT65300.017.1104621
CGGTTCG42350.016.7581359
GTATGAA415550.016.3604071
CCATAGG288850.016.2757031
TAATACT153000.016.0495834
ATAGGGT290950.015.9691183
TATGAAT413000.015.9240852
CCGCGCG166750.015.7476219
TAGAGGC301000.015.4359315
CGTAGCC120050.015.4099933
Produced by FastQC (version 0.11.5)