FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16543607
Sequences flagged as poor quality0
Sequence length88-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAG14300.021.4935055
TTAGGCA64750.018.9873124
GCTTAGG55450.018.8460642
CGCGTGT29750.018.4433631
CTTAGGC69150.017.7107733
TAGCCCC34200.016.729745
TAGGCAG78400.016.5258735
GGCTTAG71450.016.153131
CGACCCG61550.015.902755
TGTGCGT62950.015.5490748
GTATTAG36400.015.33379651
GCGTAGC34450.015.2357452
CTACACT65700.014.0345724
GTGTAGG99300.013.9092161
TGTAGGA92300.013.8834542
GTGCGTC76300.013.8820449
TCTATAC19400.013.1620023
TTCGCGC19950.012.7991398
TACACTG73900.012.6692435
GTGTGCG72550.012.6442847
Produced by FastQC (version 0.11.5)