FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28923728
Sequences flagged as poor quality0
Sequence length85-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA326280.11280703510972029No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT603300.022.50842794-95
GTATTAG107700.022.2558921
TATTAGA85150.020.9318662
CGACCCG102600.019.7209995
GTGTTAT182800.019.0867921
TAGATAG26600.018.48355
TGCCGTC762150.018.10326894-95
CGCGTGT68750.017.879421
TGTTATG185200.017.792752
GTTATGC186050.017.3295463
CCGTCTT505300.016.1196294-95
TAGGGTC118250.015.911624
GTATTGG135800.015.875151
ATAGGGT125950.015.6895543
CCGCGCG141600.015.459289
TTATGCC211850.015.3752874
GTATGAA212200.015.3506431
CATAGGG143450.015.0284972
TATGAAT208900.014.5067732
ATGCCGT781550.014.29732892-93
Produced by FastQC (version 0.11.5)