FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28923728
Sequences flagged as poor quality0
Sequence length44-101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTTAAAAGTA326490.11287963985831978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA89400.023.3869512
GTATTAG110400.023.1106821
GTGTTAT182650.020.4479791
CGACCCG108000.019.096345
TGTTATG186800.018.8908212
GTTATGC185750.018.4627573
CGGTGGT462150.018.39337294-95
TCGGTGG591950.017.54373694-95
TTATGCC210550.016.9171474
GTATTGG141450.016.2974281
TAGGGTC117650.016.2433954
TAGATAG30000.016.2406485
GTATGAA212350.015.403481
GTATCAT79600.015.16782394-95
CTACACT94250.014.9562364
ATAGGGT128200.014.9066733
CATAGGG144600.014.851712
TATGAAT208000.014.6684852
CGTATCA50100.014.60892894-95
ACCCGCG123050.014.6079537
Produced by FastQC (version 0.11.5)