FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24075454
Sequences flagged as poor quality0
Sequence length87-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCCC76950.028.34465
TAGGCCC50700.024.8228554
GCGTAGC88500.023.7905122
CGTAGCC96650.023.2525083
GCCGTCT615300.022.75778694-95
AATACGG38850.021.190265
GTATTAG58400.020.6593281
AATTTAA97450.020.3917522
TAGATAG24500.019.6975465
TTAGAAC62400.018.803783
ATTTAAG105900.018.7195823
TGCCGTC792350.018.49582194-95
AGTAGGC62650.018.4270522
GAATTTA112800.018.3299471
AAGCATA107950.018.2325677
CGCGTGT48750.018.1491321
GTGCGTT77050.017.77672632-33
TATTAGA45050.017.3291072
ATACGGA47600.017.1955976
GTAGCCC129650.017.1515524
Produced by FastQC (version 0.11.5)