FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35062025
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG429240.12242304886839822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAACG25000.013.9118953
CGACCTA32800.013.7559954
GCGATTT73850.012.7920594
TAGGTCG18700.012.0640793
CATTCGG58900.011.8097584
TTAGCGG31750.011.250319
CGGCGAA34400.011.0729831
GCATTCG58450.011.01617053
CTCGATT58050.010.93016648-49
CTTAGCG32450.09.8489258
TTCTCGA67050.09.53312746-47
GAACGCG39150.09.2438655
ACGCACG39600.09.02013452-53
TACGCAC55300.09.0089739
GGCGAAC40000.08.9300482
TCGCGCG25450.08.8643738
TACCGGT32600.08.7223444-45
GCGTCGT197300.08.62335194
GTACGCA42600.08.60557650-51
AGTCGCG28750.08.5008066
Produced by FastQC (version 0.11.5)