FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35062025
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG20700.021.5696540-41
ATCGCGT18050.017.44564842-43
GTATCGA35350.014.359014
CGTATCG43550.014.1375223
CGCGTAT13050.013.50548844-45
CCCGTAT62700.012.5274451
CCCGTCT156850.012.3245451
GTCCGAT32250.011.8132361
CCGTATC70750.010.8958112
TCGACCG20250.010.0962930-31
TATGCCG35250.09.79981148-49
TCTACTA232400.09.4847727
CCGGAAT59600.09.3910531
ACGTTCG23600.09.3600036
GTCGTTT39300.09.2153471
CGCTATT28250.09.1570971
AATCTCG114050.09.10724140-41
TCGTATG30250.09.08908244-45
CTCGAGT68000.09.0609961
CGATTGG31200.09.03830054
Produced by FastQC (version 0.11.5)