FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32886772
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG394240.11987798620065235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA34950.017.0785264
CGGCGAA32450.014.0577271
GCGAACG26150.014.0189913
TTAGCGG27250.013.1081379
CTTAGCG30550.012.7691148
TAGGTCG17500.012.3541723
GCGATTT60300.011.3017294
CGACCGT26250.010.928451
GGCGAAC39600.010.3259622
GCATTCG51600.010.2014573
GAACGCG37250.010.0938675
CATTCGG48000.09.9874084
TACCGGT30850.09.97888444-45
ACCGGTA33500.09.049211544-45
TACGCAC70850.08.9554579
TAATTCG22350.08.93727814-15
CGCGAGT39150.08.8891041
CTCGATT51850.08.83793948-49
TGACGAC72250.08.7221791
GACGACC90900.08.3764032
Produced by FastQC (version 0.11.5)