FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32886772
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT15850.023.42544244-45
ATCGCGT18900.022.38052742-43
TATGCCG50950.019.64833348-49
TCGTATG37950.016.6571444-45
CGTATGC58350.016.27049646-47
GTATCGA40100.015.4710754
CGTATCG45150.014.9897953
GATCGCG37350.013.46427940-41
CCCGTAT64650.012.9494951
CTCGTAT51900.012.5874444-45
GCGTATG31000.012.28042144-45
CCGTATC71650.012.2678332
TGATCGC41200.012.20608840-41
CCCGTCT153150.011.8848951
GTATGCC87500.011.816936546-47
GTCCGAT30200.011.0573951
CGCTATT21750.010.5959091
TATCGAA67500.010.5835355
ATCGAAG89450.010.5084966
CGGGTAT34400.09.8440871
Produced by FastQC (version 0.11.5)