FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32917070
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG415820.12632351542831727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA32100.016.8377954
GCGAACG24750.013.1028673
TAGGTCG16850.012.2728433
CGGCGAA34100.011.4486081
GTCGTAA21250.011.2798595
GCGATTT58000.011.10163694
CGACCGT25650.011.0025391
TACCGGT29350.010.72906244-45
TTAGCGG29750.010.4267619
TCGTAAG26500.09.9319516
CTTAGCG30950.09.8706368
GGCGAAC38300.09.5721462
GAACGCG39400.09.5430285
GCATTCG51650.09.3725823
CGCGAGT36350.09.1871951
CATTCGG50150.09.1843324
ACCGGTA32900.08.99994244-45
CGGTATT35400.08.89542346-47
GGTCGTA36050.08.8653544
TCGCACG28650.08.8585289
Produced by FastQC (version 0.11.5)