FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36943324
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG923520.24998291978274612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTCG24200.018.061813
GCGATTT92300.017.41483194
GTCGACT34700.014.6363211
CTCGATT78700.014.18356948-49
ATTCCGT89550.012.517467552-53
TTCTCGA89650.012.5035946-47
TCACCCG167450.012.434043
TAGCATA105650.011.7443279
ACCCGGC173400.011.5194985
CGACCTA37300.011.3403734
ACGCACG54250.011.30588252-53
CGTTCTT92850.011.21216976-77
CACCCGG180750.010.8690524
GTACGCA57800.010.77415450-51
CGGCCCG194850.010.6131918
CGGTACA64100.010.30185660-61
TCTCGAT93150.010.19213548-49
CGACCGT27300.09.8186071
TACACGC88600.09.8137952
TCGATTC96300.09.736750-51
Produced by FastQC (version 0.11.5)