FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39056662
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG539450.1381198423971818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA41950.017.2537144
CATTCGG68750.015.0398624
GCATTCG71650.014.5623223
GCGAACG30900.013.6891933
TACGCAC57650.013.2071639
GCGATTT75650.011.058722594
TACCGGT38500.010.80382344-45
CGGCGAA45050.010.1259321
GTCGTAA26500.010.1093415
TTAGCGG35550.09.7833149
GGCGAAC47650.09.5678382
TAGGTCG22400.09.4418763
CTTAGCG35100.09.3788361
CGCGAAT12700.09.2575131
TCGTAAG35250.09.0665836
ACCGGTA41150.09.023025544-45
GAACGCG51100.09.0136165
CGTGTAG263250.08.98035394
GCGTCGT376350.08.954082594
TGACGAC86150.08.9525471
Produced by FastQC (version 0.11.5)