FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39056662
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG21400.016.69130340-41
CGCGTAT14900.016.24466944-45
ATCGCGT19200.014.80962342-43
TATGCCG47350.013.89625948-49
CGCTAAT16250.012.1563711
TCGTATG37750.011.95209844-45
CCCGTCT185900.011.5370161
GTCCGAT39550.011.4164931
GTATCGA36650.011.413164
CCCGTAT66250.011.2170641
CGTATGC57450.011.207846-47
TCTACTA233800.010.7949267
CGTATCG40700.010.739363
CCGGCAT86200.010.3124661
CTACTAA234250.010.07195958
ACGACCT117400.09.84820732-33
GTCGAAT40150.09.6058611
CTCGTTT79250.09.3176931
CGATTGG39400.09.3043984
TACTAAA270500.09.2782039
Produced by FastQC (version 0.11.5)