FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39097367
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG572620.14645999051547384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA43250.016.4090674
CATTCGG71600.015.950944
GCATTCG71850.014.8488263
GCGAACG30400.014.3781293
TACGCAC60400.012.6836349
TTAGCGG36450.012.2495219
CTTAGCG37200.012.0025558
GTCGTAA25550.011.7728635
CGGCGAA44000.010.9034621
GCGATTT75450.010.52738494
TACCGGT40550.010.08378744-45
TCGTAAG33500.09.8207866
ATTCGGG125700.09.497115
GGTCGTA43100.09.4871334
ACCGGTA42350.09.10029944-45
GGCGAAC49750.09.0695942
CTCGATT67950.08.92268348-49
AGCGTCG465150.08.84113694
CGTGTAG270900.08.83083594
TAGGTCG19700.08.8273133
Produced by FastQC (version 0.11.5)