FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27657070
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGT18250.025.75300642-43
GATCGCG35250.023.13301840-41
CGCGTAT11400.019.78915244-45
TATGCCG29600.018.81557348-49
GATCGGA271900.017.5395951
TCGGAAG279900.017.0769233
ATCGGAA282650.016.9622552
TCGTATG26650.016.84212944-45
CGGAAGA294750.016.455744
GGATCGC52000.016.13343440-41
CGTATGC35750.015.64450146-47
GAGCACA335700.015.0224229
ACGTCTG161800.014.95993414-15
ACACGTC168300.014.43767412-13
AGAGCAC362200.014.0660598
CGTCTGA173450.013.91481216-17
AGGATCG64900.013.57843538-39
CACGTCT184400.013.1264514-15
CGCGTTT15400.012.66537844-45
CTCGTAT40200.012.33437644-45
Produced by FastQC (version 0.11.5)