FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27592000
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG607650.2202268773557553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT62000.017.28369794
CTCGATT54850.014.52409148-49
ATTCCGT60550.013.00147352-53
TTCTCGA65200.012.32665346-47
TCACCCG117100.011.2381053
TAGCATA68500.010.7721369
CGTTCTT72550.010.72150376-77
GTACGCA37750.010.58267650-51
ACCCGGC122550.010.5465725
TACGCAC42000.09.73561750-51
ATAGCTC87050.09.58347638-39
TCTCGAT63850.09.532454548-49
AACGTAC25400.09.4368795
TGCGAAT43300.09.2805474-75
ACGCACG40850.09.26180752-53
CACCCGG136500.09.158854
GTAGGTC45450.09.1002172
GTAGCAT89850.08.9971118
TCGATTC68500.08.88531350-51
TAGGTCG14950.08.8025553
Produced by FastQC (version 0.11.5)