FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35543855
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1001610.2817955452496641No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT84000.015.66657194
CTCGATT74050.012.94792948-49
TTCTCGA74750.012.82667746-47
GTTAGTT86650.011.9940991
CATTCGG55800.011.3708824
TAGGTCG16800.011.19039253
ACGCACG54350.011.11215252-53
GTACGCA55400.010.98638750-51
TCACCCG158800.010.9212033
TAGCATA112200.010.7655179
GTCGACT33100.010.6555821
TACGCAC60200.010.61786750-51
CGGTACA61150.010.37607660-61
GCATTCG63550.010.1321043
ACCCGGC168850.010.1319945
GCCGGTA63300.010.02363558-59
CCGGTAC63150.09.82418260-61
ATTCCGT100200.09.52188552-53
CGACCTA27850.09.4505573
CACCCGG181650.09.3921674
Produced by FastQC (version 0.11.5)