FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33605057
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA31950.019.1234594
GCGATTT79700.017.16047594
ACGCACG49400.014.12841752-53
TAGCATA99500.013.7928439
GTACGCA52800.013.30765750-51
CTCGATT69750.013.10599548-49
GCGAACG25500.013.0861593
TTCTCGA79050.011.68302446-47
CATTCGG50300.011.6798164
CGACCGT25250.011.54727651
TCACCCG149500.011.4119393
CCGGTAC63750.011.39055560-61
CGGCGAA33850.011.1142621
GTTAGTT88150.011.096611
GTAGCAT128100.011.0803088
CGGTACA65800.010.96425360-61
TACGCAC62450.010.91264250-51
AGTACGC66350.010.90873648-49
CTTAGCG31200.010.5447798
ACCCGGC160900.010.4281235
Produced by FastQC (version 0.11.5)