FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33637453
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA32250.018.653994
GCGATTT78050.017.8242694
ACGCACG45950.013.75718252-53
CTCGATT68900.013.19952648-49
GCGAACG23300.012.7079673
GTACGCA52650.012.31898850-51
TAGCATA99650.012.3099049
TTCTCGA75700.012.10698446-47
TTAGCGG31350.011.9934439
CATTCGG49100.011.8694794
TCACCCG147550.011.5626893
CGGCGAA34850.011.0673471
CTTAGCG31850.011.0673418
CCGGTAC61300.010.92572860-61
ACCCGGC160850.010.8111565
ATTCCGT88800.010.16204252-53
TAGGTCG17600.010.1475773
TACGCAC64500.010.1285339
GTAGCAT125550.010.0699438
GCATTCG62300.09.8826793
Produced by FastQC (version 0.11.5)