FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28541760
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG15250.027.73721340-41
TATGCCG41650.025.2768448-49
TCGTATG32000.023.20581844-45
CGCGTAT15450.022.96720944-45
ATCGCGT17350.022.077442-43
CGTATGC50950.020.70913546-47
CTATCGC22700.018.63403140-41
CTCGTAT46400.016.6117644-45
TATCTCG55550.016.5406640-41
TACTATC81650.016.3187738-39
GTATGCC73600.014.43179746-47
CGTATCG37650.013.9811723
GTATCGA28350.013.4283454
CCCGTCT133050.012.6909551
CCCGTAT42500.012.6162621
GCGTATG28300.012.45559944-45
ACTATCG35800.012.40621638-39
CCGTATC52200.012.3364472
CTACTAT111950.012.17487236-37
CGCTATT30100.011.8757721
Produced by FastQC (version 0.11.5)