FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37098652
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG50150.018.60284648-49
CGCGTAT16350.017.82230244-45
TCGTATG39750.016.49403444-45
CGTATGC60300.015.70534946-47
CGTATCG49500.015.2865873
ATCGCGT19700.015.0301842-43
GTATCGA40600.014.58593754
CCCGTAT64750.014.237531
GTCCGAT33550.013.4585121
CTCGTAT53500.012.56238644-45
CCCGTCT174750.012.3003471
CCGTATC77400.012.0244612
GTATGCC89700.010.97694346-47
TCGCGTT34800.010.80440532-33
ATCGAAG96000.010.3300186
CCGGCAT75950.010.0324171
CGATTGG35750.09.9914244
CGCTATT25950.09.9688181
CCGCTAT20850.09.9257871
TCTACTA227700.09.9075917
Produced by FastQC (version 0.11.5)