FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37115507
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG414490.11167569393569109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA36450.015.0862444
CATTCGG60050.013.7750264
GCGAACG24100.013.2612563
GCATTCG62850.012.8622223
TACGCAC54150.010.8493659
CGGCGAA37300.010.8446631
TTAGCGG31850.010.1819869
GCGATTT65100.010.03524194
CTTAGCG33050.09.8122918
ACGCACG39700.09.58932252-53
TCGCATA18150.09.5811567
GGCGAAC40850.09.5497832
TACCGGT31300.09.23476744-45
GTACGCA42650.08.98115650-51
TAGGTCG21150.08.8887853
CGACCGT27900.08.7664861
GAACGCG40800.08.2940225
ATTCGGG107750.08.2876065
CGAAGAT85200.08.28091051
TGACGAC81000.08.1296051
Produced by FastQC (version 0.11.5)