FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33322262
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT60250.014.27541394
CGACCGT30200.012.9248221
CGCGAGT43650.012.3898771
CGACCTA27000.010.7924974
CGCAAAA104600.010.3406911
CCGTCAT94500.010.1520041
TCGCATA16750.09.8208237
CTCGATT57750.09.76617548-49
GTACGCA38100.09.62196850-51
CGGCGAA31050.09.5418451
ACCGTTG39950.09.411683
GCGCTAA51200.09.36320542-43
GTCGTAA21800.09.2705745
TCGCGTC26900.09.085423
GCGAACG24100.08.9708743
ATTCCGT65900.08.91494352-53
TTCTCGA64800.08.88497846-47
CTAACCC124250.08.775798
AACGCGA22500.08.7732553
GACCGTT41150.08.6805422
Produced by FastQC (version 0.11.5)