FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33344287
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT60400.014.62898294
CGACCGT31300.014.1254331
CGACCTA24650.011.8213564
CGCAAAA103800.011.5094511
CGGCGAA29150.010.9720721
TAGGTCG16400.010.8901113
CTCGATT55000.010.639008548-49
GCGAACG25300.010.5888033
CGCGAGT43200.010.5610291
GTACGCA35300.010.45172950-51
ACCGTTG39850.010.3787943
GACCGTT40600.09.9559462
CTAACCC123100.09.8504368
ATTCCGT63150.09.60084652-53
ACGCACG39950.09.58813252-53
TCGCGTC28400.09.2674923
TTCTCGA62500.09.13675746-47
CCGTCAT89100.09.1324371
TAACCCC132500.08.8323719
GCGATAG50400.08.81239266-67
Produced by FastQC (version 0.11.5)