FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37306903
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG483060.12948273942760674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA36150.015.341474
GCGAACG28450.015.0332333
CATTCGG60150.013.752144
GCGATTT76900.013.14035694
TAGGTCG18150.012.9475223
GCATTCG67550.012.6630793
CTCGATT66100.011.16331748-49
CGGCGAA43000.010.7180371
CGACCGT30650.010.5870811
TTCTCGA74300.010.21594946-47
TACGCAC58700.010.0084129
ACGCACG49350.09.80942152-53
GAACGCG46000.09.6042465
GTACGCA50450.09.59555850-51
ATTCCGT80600.09.30077152-53
GGCGAAC46950.09.1099132
CGGTACA54600.08.82326260-61
AACGCGG54550.08.7020176
TACCGGT35350.08.57561644-45
GATCGGA635600.08.5037294
Produced by FastQC (version 0.11.5)