FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406684

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406684
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37306903
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG17700.015.93190340-41
CGTATCG49600.013.17118553
GTATCGA41350.012.9574964
GTCCGAT35100.012.5964581
CCCGTAT68000.012.0356091
CCCGTCT170900.011.2566451
CGCTAAT13750.010.9464941
CCGGCAT79250.010.7425411
CCGTATC75150.010.6957652
CTCGTAT39750.010.2946091
ATCCGGA90300.010.12329434-35
TCTACTA235700.010.0698627
AATCCGG90750.09.917726534-35
CAATCCG89700.09.85042932-33
TCGCGTT32400.09.64642432-33
CTACTAA234450.09.4419658
CGATTGG32450.09.4143514
AGTTACG45300.09.2338666
TCGACCG27500.08.972733530-31
TACTAAA269500.08.96388159
Produced by FastQC (version 0.11.5)