FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37352230
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG507110.1357643171505423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA34550.016.3239974
GCGAACG27500.014.1853063
CATTCGG62400.013.708194
GCATTCG67650.012.5748873
GCGATTT77650.012.22653194
CGGCGAA42800.011.6485481
TTAGCGG32050.011.144979
CTTAGCG33400.010.5537828
TAGGTCG19200.010.5259323
ACGCACG49500.010.39683352-53
CTCGATT67150.010.25386348-49
CGACCGT30400.010.2112761
GTACGCA53300.09.74380850-51
TACCGGT35000.09.60140544-45
GGCGAAC46850.09.5307122
TTCTCGA75100.09.32487846-47
TACGCAC62150.09.3015939
CGGTACA56650.09.00174360-61
GTCGTAA25400.08.8817975
CGCGAGT46300.08.8378781
Produced by FastQC (version 0.11.5)