FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27341034
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTTAAGATCTCGTAT300740.10999583995250509TruSeq Adapter, Index 11 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG65550.031.04603648-49
TCGTATG52300.028.62191644-45
CGTATGC73000.027.81325746-47
CGCGTAT20200.026.98967244-45
ATCGCGT21750.024.74213442-43
CTCGTAT64400.023.42664544-45
CGGCTTA91350.023.04944432-33
GTATGCC95450.021.8377946-47
GATCGCG34100.016.9528140-41
ACGGCTT128650.016.6040932-33
GCGTATG33500.016.48481444-45
CGTATCG41850.016.1718463
GTATCGA36100.016.1438474
GCCGTCT129600.015.9021450-51
GCTTAAG137050.015.7407234-35
ATGCCGT132950.015.73122848-49
CCGTATC52650.014.8198422
TAAGATC143850.014.81693638-39
CCCGTAT52100.014.80505851
TCGCGTA37000.014.734942-43
Produced by FastQC (version 0.11.5)