FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27341034
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG379920.1389559736475219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT49550.016.50443594
CGACCGT24200.012.8258171
CGACCTA21150.011.9999014
CTCGATT48400.011.60424348-49
ATTCCGT52400.010.94265852-53
GCGAACG16000.010.8686613
GTACGCA35150.010.63006950-51
ACGCACG37850.010.18221552-53
TCACCCG106650.010.0477373
CGGCGAA26100.09.729931
TTCTCGA58150.09.57773746-47
GCGTCGT257000.09.38165294
GCGATAG43350.09.2698266-67
CCGGTAC41550.09.16241360-61
CGTGTAG175050.09.12876294
TAGCATA66950.09.0559379
CGCAAAA72800.08.9792461
CGTTCTT60450.08.94123376-77
TCGATTC56100.08.88049750-51
TCTCGAT58000.08.83268548-49
Produced by FastQC (version 0.11.5)