FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27335300
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG392880.14372624408731566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT51150.014.793591594
CGACCGT21450.012.7179841
GCGAACG17350.010.8356033
CTCGATT50350.010.78145848-49
GTACGCA38000.010.76042650-51
CGACCTA23700.010.7087314
ACGCACG39300.010.64365752-53
TAGCATA69200.010.3914929
TCACCCG106950.010.1074043
TTCTCGA55600.010.05930446-47
CGGCGAA28150.09.6909691
CGCAAAA72450.09.4783351
ATTCCGT55700.09.28177252-53
GCGATAG43100.09.15998266-67
CCGGTAC45300.08.92266160-61
TACGCAC40000.08.87116650-51
GGCGATA47800.08.701864-65
CGATAGA48800.08.66792866-67
ACCGTTG31300.08.5590013
AGAGCGT401550.08.54430294
Produced by FastQC (version 0.11.5)