FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39483331
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG48950.018.96287748-49
ATCGCGT19150.015.95270742-43
TCGTATG44050.015.8975544-45
CGTATCG49300.015.7299713
GTATCGA45750.015.5123214
CGTATGC59600.015.41666146-47
CGCGTAT16600.015.00574644-45
CCCGTAT69900.014.6687351
ACGGACT109100.013.56988432-33
CGGACTT116700.012.98820832-33
CTCGTAT59400.012.30364444-45
CCGTATC76400.012.2431912
GTCCGAT35850.012.0701311
CCCGTCT177800.011.7982181
TCTACTA244000.010.8637477
CGCTAGT14450.010.7413631
GTATGCC91350.010.70149946-47
CTACTAA234500.010.4420398
AGTCACG148800.010.29711328-29
ATCGAAG103050.010.1250216
Produced by FastQC (version 0.11.5)