FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38202528
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG54550.018.30853748-49
TATCGCG17800.017.42666440-41
TCGTATG41000.017.36673744-45
CGCGTAT17450.017.2374944-45
ATCGCGT18800.016.24968542-43
CGTATGC63650.015.87557846-47
CGTATCG50700.014.1831773
GTATCGA47900.014.1291814
CCCGTAT73700.013.1469061
CTCGTAT57900.012.58179644-45
CCGTATC76300.011.7048922
CCCGTCT183700.011.7012121
GTATCGC29300.011.06806740-41
CGCTAAT17150.010.970331
GTATGCC95450.010.93116746-47
TATCTCG71350.010.73702540-41
CGGCTAT57300.010.6711831
CGCTACT26950.010.2971511
CGCTATT28950.010.2356591
GCGTATG32600.010.01974544-45
Produced by FastQC (version 0.11.5)