FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38240566
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG401720.10505074637232095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA39700.019.2969934
GCGAACG31000.016.5256043
CATTCGG65500.014.063954
TACGCAC55650.014.0195959
GCATTCG66150.013.9257553
TTAGCGG35500.013.7688469
CTTAGCG37400.013.5720268
CGGCGAA42300.013.3432711
GCGATTT65050.011.70472994
TACCGGT35550.011.70037144-45
GGCGAAC46900.011.52481652
GAACGCG47500.010.9830235
TAGGTCG19400.010.9019283
TGACGAC89950.010.24887851
AACGCGG54000.010.1832096
GTCGTAA26650.09.8760515
CGACCGT29750.09.6441571
ACGCGGA54400.09.4171647
CGAAGAT96300.09.2800161
AGAGCGT375800.08.95467794
Produced by FastQC (version 0.11.5)