FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39692688
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG46850.021.41793344-45
TATGCCG58500.019.60303148-49
CGTATGC67550.016.97671946-47
AGTTACG123600.015.8565026
TATCGCG13250.015.78462240-41
GTTACGG119000.015.6400467
TTACGGA121150.015.5564588
TTCGCGC35600.015.4463427
TATCTCG83750.015.09584940-41
CTCGTAT69100.014.62344444-45
TACGGAT138600.014.309989
TCGCGTT143500.013.64119332-33
GTCCGAT34000.013.4186831
CCGGCAT83300.013.26905251
GATCGGT152850.012.89933326-27
TCGGTCG153800.012.80437728-29
CGCTATT44950.012.7657761
CGGTACC120050.012.31254332-33
GTATCGA34100.012.2666344
TACCGCA163600.012.15200638-39
Produced by FastQC (version 0.11.5)