FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29679333
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG509640.17171544926565566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT54400.015.63776994
CGCAAAA79000.014.4060171
CTCGATT52450.011.96274748-49
CATTCGG48750.011.7619384
ATTCCGT55700.011.39127252-53
CCGTCAT77850.011.2963631
GCATTCG49450.011.0251723
TTCTCGA57500.010.70775846-47
CGCTAAT17250.010.0871451
GCGCTAA47750.09.94126542-43
ATTCGGG61450.09.7134945
GTACGCA36900.09.55276950-51
TACTCTA88050.09.4479499
ACGCACG38250.09.39990952-53
CGTTCTT66600.09.20944876-77
TCACCCG125500.09.175213
ATCGACC71950.09.14515142-43
TACGCAC38150.08.99337550-51
CGCGGGT77900.08.693221
CGGGGTT92850.08.5597381
Produced by FastQC (version 0.11.5)