FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29679333
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG57700.030.05664348-49
CGCGTAT21400.028.44106944-45
CGTATGC66500.026.29124846-47
TCGTATG43550.026.2785144-45
ATCGCGT25050.024.29695942-43
GTATGCC86400.020.6165246-47
CTCGTAT56000.020.22641644-45
GTATCGA45500.019.3161644
CGTATCG50300.019.248193
ACGCACG102300.017.87163232-33
GCGTATG35150.017.58289344-45
CCCGTAT58900.017.407731
CGCACGG107100.017.02678132-33
ACGGACA108700.016.53839336-37
CGGACAT116100.015.46402436-37
CCGTATC70000.015.4445262
TCGCGTA40750.015.10892742-43
AGTCACG123050.014.89656328-29
TCACGCA123150.014.75073830-31
GTCACGC125450.014.72397128-29
Produced by FastQC (version 0.11.5)