FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35914821
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA41050.015.570984
CGTATCG46300.015.3280223
TATGCCG41000.015.07410248-49
CCCGTAT63600.014.051451
TCGTATG36300.014.04794144-45
CCCGTCT162600.012.5543391
CGTATGC50350.012.41485846-47
CCGTATC75800.012.3402912
GTCCGAT34100.010.7588261
CTCGTAT48600.010.58930444-45
TCTACTA221100.010.2458617
CGCTAGT13000.010.1306981
CTCGTTT66950.010.1166461
CCCGTTT88450.010.10371
TATCGCG13100.010.04560940-41
CGCGTAT11900.09.87376144-45
CTACTAA219000.09.8505118
ATCGAAG97900.09.6974796
GCCGTAT41850.09.6655741
TCGCGTT29550.09.6224932-33
Produced by FastQC (version 0.11.5)