FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005406711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005406711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35914821
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG360210.10029564117833135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT78900.015.84523494
CGACCTA34500.014.0317414
CATTCGG63400.013.1213184
GCATTCG67000.012.977483
TTAGCGG33950.012.597819
CTCGATT67100.012.53789348-49
GCGAACG29700.012.02681353
TTCTCGA72450.011.74178846-47
CGGCGAA38600.011.331071
CTTAGCG37100.011.0214518
ACGCACG43200.010.77072452-53
GTACGCA45500.010.22627650-51
TACGCAC54050.09.9129439
ATTCCGT83400.09.8620152-53
TCGATTC77050.09.3938150-51
TCACCCG173200.09.2533693
GTCGTAA22500.09.1910175
TACCGGT35000.09.06420644-45
ACCCGGC177900.09.0353215
GGCGAAC42850.08.9942992
Produced by FastQC (version 0.11.5)